Dyaningtyas Dewi Pamungkas Putri

6 Questions 18 Answers 0 Followers

Questions related from Dyaningtyas Dewi Pamungkas Putri

How poly IC in transfection?

Dear all, how microliter of DNA 1ug/ml I should apply in volume of dilution medium to get the final concentration is 1ug/ml in 96 well, 24 well and 6 well Thank you for your help

30 May 2016 7,968 2 View

Does anyone have experience in neutrophil movement?

i would like to investigate the movement of neutrophil in 96 well plate and i need to coat the well. does anyone have experience about the best coat for neutrophil movement? BSA? HSA? FBS?...

25 February 2016 3,659 3 View

What is a method to investigate T cells infiltration?

I would like to investigate the T cells infiltration in several cells both in normal condition or due to cytokine/chemokine . May you help me to give some suggestion, the method to analysis the T...

25 December 2015 8,844 8 View

Why do I have problems in transfection?

i check the condition of the cells. But I get the worst condition the cells. Only a few cells that attach on TCD. so, may you help me to solve electroporation problem? thanks

30 September 2015 5,293 6 View

What is the critical step when stimulation?

in my case, may I say that the production of IL10 of the supernatant isn't enough? Is it necessary to increase the cell number? Thanks for helping regards, Dewi

12 May 2015 6,196 1 View

Does anyone have some experience in PCR with gene length about 4,000bp?

I try to PCR my gene target, that length about 4,000 bp. First time PCR, I try with cDNA 5ng, each concentration primer 0.1mikroMolar, dNTP 0.4mM, KOD FX 1.0U. and I got the smear band, I try to...

06 February 2015 7,861 21 View