Why is my enzyme loading low and what should I do to enhance the immobilization degree?

I am working on lipase immobilization on silica gel. First, I did a pretreatment for silica gel using 15% 3-APTES in acetone and stirred in 50 degree Celsius for 2 hours. I used gluteraldehyde 2%...

23 July 2013 8,075 23 View