Duygu Yeşildağ

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Questions related from Duygu Yeşildağ

How can I perform the optimization of site directed mutagenesis (single nucleotide change) with Q5 high fidelity enzyme without using a kit?

I have a plasmid with 9kb length, I am using DMSO since I heard it increases the efficiency. The PCR protocol I often use is as follows with various annealing temps such as 55, 60, and 65C; 95C 1...

09 September 2016 8,163 3 View

After treating with sonicated H. pylori G27 strain, AGS cancer cells started to die. Can anyone give me an idea about why that could happen?

We have done this experiment twice and in both of them, only the g27 treated cells die after 6 hours.

12 December 2015 8,412 3 View