Duy Le Pham

10 Questions 27 Answers 0 Followers

Questions related from Duy Le Pham

Where can we get LUVA cells?

Dear all,Would you like to suggest me where I can get LUVA cell stocks?Thank you very much,Duy

28 September 2016 6,957 3 View

What is the function of fetal bovine serum on degranulation of eosinophils, mast cells and neutrophils?

Hello all, Could you please let me know whether FBS could inhibit the degranulation of eosinophils, mast cells and neutrophils? As I saw in some reports, FBS could inhibit eosinophil...

14 December 2015 3,788 3 View

Any advice on a MTT assay false positive result?

Hello all, I am using MTT assay to test the toxicity effect of a substance on A549 cells (adherent cell). I seed 5x10^4 cells/well of a 96 well-plate, incubate overnight for cell attachment. Then...

06 June 2015 6,712 9 View

How should one interpret LC3 puncta in immunofluorescence?

Hi all, I am trying to detect autophagy in primary blood neutrophils using LC3 puncta as markers. I have attached 2 figures with different morphology of LC3 distribution (green color). I...

21 November 2014 3,205 18 View

Does pressing hard when mounting cause cell morphology changes?

Hello all, I stained the cells (LAD2) for immunofluorescent microscopy and mounted the slides by mounting medium with DAPI (Vectorshield). When mounting, I pressed the cover slips against the...

02 September 2014 1,278 2 View

What is a specific marker for neutrophils to distinguish them from other granulocytes in peripheral blood (including eosinophils and monocytes)?

Thank you very much!

27 August 2014 183 9 View

I got 2 bands for beta-actin in Western Blot when I used human neutrophils, but not for other cell lines. How can I solve this problem?

When I performed WB with cell lysate from human neutrophils, I got 2 bands of beta-actin. For other cell lines (HMC-1, LAD2 cells) I just only got 1 band for b-actin. I used the same antibody and...

20 April 2014 5,250 1 View

Skimmilk as blocking buffer for ELISA?

Hi all, Can Skimmilk be used as blocking buffer for ELISA tests with human serum samples? Can anti-milk-IgG or IgE antibody in serum samples interacts with skimmilk that blocks the plate? Thanks...

11 February 2014 9,837 18 View

Multiple fragments cloning (In-fusion Cloning kit)

I am trying to clone 2 fragments of 1.6 and 1.1kbp into pGL3 basic vector (4.8kbp) using In-fusion HD cloning kit (Clontech). I added totally 90ng of 1.6kbp fragment and 70ng of 1.1kbp fragment...

12 August 2013 4,067 10 View

Choosing a colony containing pGL3 with the insert.

I did ligation steps to get pGL3-basic vector with the insert, then transformed into competent cells and cultured on an agar plate with ampicillin. After incubating, I had to choose colonies for...

27 March 2013 4,513 14 View