21 Questions 49 Answers 0 Followers
Questions related from Duy Le Pham
Dear all,Would you like to suggest me where I can get LUVA cell stocks?Thank you very much,Duy
28 September 2016 7,011 3 View
Hello all, I would like to ask whether you have experienced that IL-8 could induce ROS production from neutrophils without priming step? I tested two times (treating neutrophils with IL-8 100...
18 April 2016 5,338 5 View
Hello all, Could you please let me know whether FBS could inhibit the degranulation of eosinophils, mast cells and neutrophils? As I saw in some reports, FBS could inhibit eosinophil...
14 December 2015 3,851 3 View
Hello all, I am using MTT assay to test the toxicity effect of a substance on A549 cells (adherent cell). I seed 5x10^4 cells/well of a 96 well-plate, incubate overnight for cell attachment. Then...
06 June 2015 6,775 9 View
Hello all, I am trying to induce NET induction by IL-8 (100 ng/ml) using healthy neutrophils and patients' neutrophils. I quantify the NET induction by Picogreen assay. In case of healthy...
29 May 2015 8,045 4 View
Hi all, I used lentivirus containing MISSION shRNA of ATG5 gene to produce a stable cell lines after puromycin selection. Now I would like to knockdown another gene (Beclin-1) using the previous...
17 March 2015 6,480 3 View
Hello all, As I know, IL-8 can triggers neutrophil degranulation and therefore induces the release of MPO into the culture supernatant. However, I could not find any study investigating the direct...
22 November 2014 4,270 6 View
Hi all, I am trying to detect autophagy in primary blood neutrophils using LC3 puncta as markers. I have attached 2 figures with different morphology of LC3 distribution (green color). I...
21 November 2014 3,266 18 View
Dear all, I am doing immunocytochemistry with HL-60 cells. I tried to detect LC3 puncta for autophagy investigation. I used mounting media containing DAPI from Vectashield (aqueous form). All the...
20 October 2014 3,282 8 View
Hello all, I stained the cells (LAD2) for immunofluorescent microscopy and mounted the slides by mounting medium with DAPI (Vectorshield). When mounting, I pressed the cover slips against the...
02 September 2014 1,340 2 View
Thank you very much!
27 August 2014 258 9 View
Hello all, I would like to knockdown some gene in LAD2 cells to produce a stable expressing cell line. Could you suggest me an appropriate method to do? Because LAD2 cells are stem cells and they...
27 June 2014 8,466 0 View
When I performed WB with cell lysate from human neutrophils, I got 2 bands of beta-actin. For other cell lines (HMC-1, LAD2 cells) I just only got 1 band for b-actin. I used the same antibody and...
20 April 2014 5,346 1 View
I tried to isolate neutrophil from human venous blood followed the protocol as below: 1. mix 10ml blood with 10ml Hank's balance salt solution (HBSS) without Ca and Mg. Stand the tube for 30 min...
04 March 2014 8,499 27 View
Hi all, Can Skimmilk be used as blocking buffer for ELISA tests with human serum samples? Can anti-milk-IgG or IgE antibody in serum samples interacts with skimmilk that blocks the plate? Thanks...
11 February 2014 9,920 18 View
I am trying to clone 2 fragments of 1.6 and 1.1kbp into pGL3 basic vector (4.8kbp) using In-fusion HD cloning kit (Clontech). I added totally 90ng of 1.6kbp fragment and 70ng of 1.1kbp fragment...
12 August 2013 4,124 10 View
What method do you usually use for transfection with human mast cell (HMC-1 cell line)? Does Lipofectamine work well for that cell line?
18 May 2013 9,663 2 View
I tried to ligate my insert (520bp) into TOPO vector (TA cloning). My PCR product has concentration of 30~40 ng/mcl I used the mixture as below: PCR product 3 mcl TOPO vector 0.3...
24 April 2013 2,810 4 View
I have 2 constructs and need to check the promoter activity by luciferase assay. I finished cloning and now have the bacteria which contains pGL3 basic vectors including inserts. The next step is...
02 April 2013 7,965 9 View
I did ligation steps to get pGL3-basic vector with the insert, then transformed into competent cells and cultured on an agar plate with ampicillin. After incubating, I had to choose colonies for...
27 March 2013 4,605 14 View
When I did cloning, after getting the PCR product of the target sequence, I inserted it into "TOPO I" vector and then transformed into competent cells. The next day, I chose two colonies from the...
26 March 2013 9,592 3 View
