Dushyant Kumar Garg

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Questions related from Dushyant Kumar Garg

What is the best way to dissolve zinc free insulin and prepare zinc-insulin hexamer?

I am working on human insulin and need to start experimenting with 1) monomeric insulin and 2) hexameric insulin. The insulin is known to form hexamer in the presence of zinc ions. To obtain...

02 February 2018 5,268 0 View

Protein undergoes initial compaction and structural gain at low guanidine HCl concentration before unfolding at higher GdnCl concentration

We studied guanidinium hydrochloride (GdnCl) mediated equilibrium unfolding of a multidomain dimeric hyperthermophilic protein (each subunit consists of 2 domains). Upto 1M GdnCl, protein exhibits...

03 March 2016 1,657 8 View

How do I make sure that the pH of my protein sample is not altered at different guanidine concentration?

I want to study the stability of one dimeric hyperthermophillic protein at different pH. One way of doing so is to subject pH incubated protein sample to thermal scanning and determine Tm. The...

12 December 2014 4,194 3 View

How can I calculate the dn/dc value for a protein in buffers containing high concentrations of urea/guanidine hydrochloride?

I am working on a hyperthermophilic protein which is dimeric in its native state. I am checking at what guanidine concentration the dimer dissociates into a monomer. One way to do so is to employ...

06 June 2014 8,089 7 View

Is it possible to observe emission max of intrinsic tryptophan fluorescence exceeding 360 nm?

I am studying conformational change in a peptide and monitored λmax after exciting it at 295 nm. The peptide was dissolved in 20mM glycine HCl buffer (pH 2.4) and it contains single tryptophan. At...

04 April 2014 5,760 3 View