I added Akaline Phosphatase and ligase buffer into my linearized plasmid without ligase but there are a lot of colonies after transformation.how come?

I am trying to find out what are the problems in my ligations. The first test: linearized Plasmid+ AP+ ligase buffer, after transformation, it gives a lot of colonies ! The second test:...

29 July 2019 8,620 3 View