Dunja Mrdjen

4 Questions 7 Answers 0 Followers

Questions related from Dunja Mrdjen

Does anyone know of a mouse antibody for the MHCII-bound antigen peptide?

I'm looking for an antibody either for flow cytometry or histology that recognizes the peptide antigen bound to MHCII on mouse dendritic cells. I want to track antigen transport to the skin...

28 October 2014 1,823 6 View

Does anyone know a longevity assay for murine dendritic cells?

I am referring to conventional dendritic cells from the mouse lymph node, not bone marrow derived dendritic cells. I don't know what to put in the culture medium. GM-CSF will result in DC...

21 July 2014 3,837 7 View

Adoptive transfer EAE in C57BL/6 mice not working!

I am trying to induce passive EAE by adoptive transfer of primed T cells from WT C57BL/6 donors to WT C57BL/6 recipients. The protocol I followed was: Day 0 - immunised donor mice with 200ug...

15 July 2014 1,341 10 View

Should I use male or female C57BL/6 mice for EAE experiments?

I have performed some pilot experiments in male and female C57BL/6 mice and think I might see differences based on sex of the mice. Does anyone know if sex plays a role in disease onset and/or...

14 July 2014 1,337 3 View