Duncan Wells

3 Questions 3 Answers 0 Followers

Questions related from Duncan Wells

Two different primaries work well, but expected to work effectively in double-immunostaining?

If I have 2 primary antibodies (one raised in mouse, other in rabbit) that I have found to work well individually on whole mounts, would it be expected that these 2 would work as effectively in a...

03 March 2018 5,426 3 View

Obtaining a gut microbiota population?

I would like to obtain a gut microbiota population (ideally sheep or cow) for testing it as a supplement for a parasitic species I am trying to culture. As I am not a microbiologist, I know very...

30 June 2017 1,067 4 View

When using a TRIzol extraction, I have consistently got low levels of RNA and poor quality values. Any suggestions?

I am working with low (and expensive) amounts of tissue. Following a TRIzol extraction, I have got poor quality RNA and a low amount. I think that the main issue would be following the isopropanol...

05 November 2016 9,568 3 View