Dudley William Lamming

3 Questions 2 Answers 0 Followers

Questions related from Dudley William Lamming

Px330-based constructs are not growing well in Stbl3 bacterial cells - anyone have similar issues?

We have at least 1 construct that a) works in mammalian cells and b) seems to be cloned properly by PCR digest, but that is growing very poorly in Stbl3 cells (10x less plasmid and lower OD than...

05 May 2015 6,693 2 View

Why am I getting a low mouse hepatocyte number (but high viability)?

I am perfusing mice to isolate primary mouse hepatocytes, and getting good viability but low cell numbers (like 5-10 million live cells per liver with 50-70% of the cell viable). The procedure...

07 July 2014 3,353 26 View

Has anyone ever had EDTA that acted as a base and easily dissolved?

EDTA is of course an acid, and it is well known that preparation of 0.5M EDTA requires the addition of sodium hydroxide. I obtained EDTA from a stock room ( 2 different bottles, 1 sealed, but...

02 February 2014 7,863 7 View