Duc Anh Nguyen

4 Questions 11 Answers 0 Followers

Questions related from Duc Anh Nguyen

Why do i get two peaks on my HPLC run?

Hello all i have a problem. I recently got a new RP-HPLC column with a guard column as well, after connecting them and starting the first runs i noticed that my sample (70% purity) elutes into two...

01 August 2023 199 17 View

HPLC Chromatograms gets worse, what could be the reason?

Hello fellow researchers, i started to run several HPLC methods to quantify an enzyme activity. I incubate my Enzyme (different mutants) with my substrate (futalosine, quite hydrophobic). To...

22 June 2023 4,169 5 View

Different retention times of the same substrate within an enzyme reaction and without, what can be the reason?

I am currently running an HPLC to quantify the activity of my enzyme. I incubate it with my substrate which shows a peak corresponding to its product and also the substrate peak which wasnt...

20 April 2023 292 3 View

Serine Residue deprotonated at a pH of around 7, what can be the reason?

Hello all, i work on a protein which is a hydrolase and after experiments including pH titration via NMR we discovered, that one serine residue (in the active site) deprotonated at a pH of around...

17 October 2022 4,175 0 View