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Questions related from Draven Rane
Hello, I would appreciate getting help to do appropriate statistical analyses on my qPCR data in GraphPad Prism for the sake of the statistical tools and graphing power. I had already done the...
20 October 2022 5,496 1 View
Hello, I am new to microscopy and have recently executed my first experiment using RNA FISH probes to target various vRNA segments of IAV within A549 cells. The virus has been engineered to...
23 March 2021 451 7 View
I have inserted a plasmid (using a pET28a+ backbone, expressing the lac0 operator) into chemically competent BL-21 DE3 cells. I had selected for kanamycin and plated. I then picked a colony and...
17 September 2019 6,294 3 View
I know the methods available for transforming bacteria in order to take up foreign plasmid DNA. I, however, would like to utilize a non-viral method to infect my bacteria with foreign RNAs. I have...
20 August 2019 3,430 2 View
Hello, I have been working with Gibson Assembly in order to create three separate plasmids. I have ran PCR on pET28a+ and an already cloned plasmid containing two genes of interest. The GC...
06 August 2019 5,786 6 View
I used a proof reading DNA polymerase, Q5, to amplify backbones and inserts that must have correct nucleotide sequences. I have already excised the bands at the appropriate lengths and have...
29 July 2019 4,136 3 View
The answer may be as obvious as there must be something wrong with the plates, but I am still perplexed. I have a plasmid system which has kanamycin resistance on the plasmid vector while...
15 July 2019 1,714 5 View
