Dr. Sonali Dalwadi

4 Questions 12 Answers 0 Followers

Questions related from Dr. Sonali Dalwadi

How does the density gradient separator like Percoll separate the cells? What is the mechanism behind it? Is it fixed for number and types of cells?

I am working on drug targeting studies. I want to check the amount of drug uptake. From total cells first I want to separate and collect the cells which have taken up the drug. The method...

08 August 2013 4,414 7 View

Can anyone tell me the lag time of primary macrophage cell line survival?

I am working on primary cell culture of alveolar macrophage which were isolated by BALF method. For targeting study the cells are required to adhere on plates. During that incubation period the...

11 November 2012 6,424 0 View

Primary cell culture of alveolar macrophages done by Bronchoalveolar lavage fluid method, how can the RBCs present in isolated cells be removed?

I want to prepare alveolar macrophage cell culture for phagocytosis study. During isolation of alveolar macrophages from rat lungs, the few RBCs are also present. I need to remove that before...

09 September 2012 2,922 10 View

For drug -uptake studies using macrophage cell line, Is opsonization of drug being tested necessary prior drug treatment step?

We are doing drug - uptake studies using macrophage cell-line specific for evaluation of phagocytosis of drug. Does anyone know whether opsonization of product to be tested for uptake studies is...

09 September 2012 7,009 3 View