Dr Saurabh Malkar

4 Questions 12 Answers 0 Followers

Questions related from Dr Saurabh Malkar

Are you familiar with future directions for low/negative estimates for small RNA estimation?

I am measuring levels on mirco RNAs in a murine myoblast cell line. So, I collected the cells and processed them for small RNA extraction using MirVana Kit. Upon estimating the concentration and...

05 May 2015 8,837 9 View

How can I prevent contamination in the cell culture?

I wipe down my work area in the BSC with 70% EtOH, wear PPE, spray my gloves with EtOH and follow good handling measures while performing passage or any other cell culture protocol. Yet, I had a...

04 April 2015 1,506 11 View

Why shouldn't one add serum to obtain conditioned media?

I am not sure why one shouldn't add serum to the media that we add to cell cultures to obtain conditioned media the following day. Could someone help me get up to speed with this? Thanks.

04 April 2015 4,684 5 View

How do I normalize the controls for miR 486 in C2C12 cells?

I am assaying (RT qPCR) the levels of miR 486 in C2C12 cells across different serum concentrations and am looking for different normalisation controls. I tried miR 30d, but it showed a lot of...

04 April 2015 4,676 5 View