Dr. Siddabasave Gowda B Gowda

6 Questions 14 Answers 0 Followers

Questions related from Dr. Siddabasave Gowda B Gowda

How do you normalize the LC/MS peak area by internal standard ?

Well, the amount of IS on the column was 10 ng. So the amount in the sample can be calculated by simple equation C analyte = (10/A) IS *A analyte. However, this amount will be divided by the...

22 June 2021 8,381 6 View

Does anybody know the interpretation of IR spectrum of Merrifield resin. Why always there is a broad band around 3400 cm-1?

Please assist me with the help of the reference attached.

21 August 2017 2,317 4 View

Is there is any crystallographic data /PDB structure available for almond ß-glucosidase ?

I would like to study the enzyme substrate interactions. But I could not find the protein structure of almond ß-glucosidase in the literature. If anbody have any idea concerning its structure...

22 February 2016 3,813 4 View

What is the O.D range in an MTT assay ?

I am experiencing very low O.D values in MTT assay, when I measured using microplate reader at 570 nm, even though I took enough no of cells there is no much increase in O.D. is it quite common or...

12 August 2015 4,199 13 View

How can we separate E/Z isomers no UV nor fluorescent?

Hey, I am stuck in the separation of sphingoid base isomers (E/Z), is there is any efficient technique to do this?

25 April 2015 4,797 6 View

What is the best method for the analysis of sphingoid bases?

Even though GC-MS/ LC-MS are reported, its quite difficult to do the analysis by HPLC with OPA derivatization. If anyone has an idea about separation and analysis of sphingoid bases please share...

05 March 2015 4,842 7 View