I am getting similar protein band in uninduced and induced culture. Please suggest?

The protein is an enzyme cloned in pET28a vector. I have used IPTG conc.(0.5mM, 0.8mM, 1mM) and culture at different time point (4h, 6h and overnight). I checked its induction 6 months back when...

29 February 2016 5,289 5 View