Dr. Pankaj K. Bagul

3 Questions 14 Answers 0 Followers

Questions related from Dr. Pankaj K. Bagul

Where do I get oligonucleotide probes for EMSA assay? How to design the probes for transcription factor of interest?

I am planning to do EMSA assay with my samples for some known and some new transcription factor. How do I get the oligonucleotide probes for the assay? Somebody please tell me where I will get...

17 July 2013 9,002 1 View

Use of Coomassie blue stain and ponceau stain as loading control in western blot?

Is it reliable to use coomassie staining (before transfer) or ponceau staining (after transfer) as a representation of loading control? As most people get problems in selecting a good loading...

26 April 2013 9,252 27 View

Can anybody please tell me the rules or ethics behind publishing western blot results?

It seems to be very difficult to follow a proper pattern of publishing the western blot data. Everybody follows different patterns (ex. in the no. of samples or etc.). Somebody please tell me...

21 February 2013 7,228 3 View