Dr Dhanashree

2 Questions 8 Answers 0 Followers

Questions related from Dr Dhanashree

Can anyone help with my 2.2 kb amplification?

I have amplified 1.04kb and 1.16 kb fragments, then I went for touch down PCR by using these two fragments as template to amplify the 2.2 kb of my desired gene. I got amplification of 2.2 kb with...

05 June 2014 6,086 2 View

Why wasn't I able to get clones even after treating with CIP?

I believe vector ligation is the main problem. I'm using pBluescript for cloning and my insert around .8kb and 1.3 kb fragments but unfortunately I'm getting more background and due to that I...

14 May 2014 2,444 21 View