Nanodrop 2000c giving variable results for Ab-dye conjugate while using 1.5ul, 2ul and 3ul volume, why?

Hi, we use 2000c Nanodrop to estimate antibody-PE conjugate. We use UV vis method in ND2000c. We see different Abs values at 280nm and 565nm for 1.5ul, 2ul and 3ul. We have an excel which...

20 June 2021 9,446 3 View