How much poly-A RNA should I use for transcriptome sequencing using minION (ONT)?

I extracted ~87 ug of total RNA from mushroom samples using TRIzol (Invitrogen) and I got ~1,2 ug of poly-A RNA using the Oligotex kit (QIAGEN) for mRNA purification. The protocol of direct cDNA...

01 April 2021 2,070 0 View