Douglas Vendas Faget

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Questions related from Douglas Vendas Faget

Does anyone has a protocol to stimulate T cells with antigen-pulsed irradiated splenocytes?

I would like to know how many splenocytes should I use for each T cell. When T cells are stimulated with differentiated DCs, I've seen papers doing 1:1, 1:5 and 1:10 (DC:T cell). However I...

26 August 2019 6,150 2 View

Is it possible to dephosphorylate proteins in a fixed tissue upon phosphatase treatment as a control for phospho-protein staining by immunohistochem?

I am running a immunohistochemistry (IHC) for a specific phospho-protein and my PI asked for a control that should be the same tissue treated with phosphatase prior the staining just to confirm if...

18 December 2018 7,068 5 View

Does anyone know a commercial kit or any substance that I can use to enhance adenovirus transduction NIH3T3 cells?

I'm having trouble to transduce NIH3T3 with purified adenovirus serotype 5 (Ad5). I looked for information in the internet and I found some commercials kits available for adenoviral transduction...

21 January 2015 550 5 View

Which free software or websites are good to do a promoter analysis to search for putative transcription factor binding sites?

I would like to know which free software or websites are good to do a promoter analysis to predict putative transcription factor binding sites?

31 July 2013 9,779 2 View