Dongxue Hu

5 Questions 6 Answers 0 Followers

Questions related from Dongxue Hu

How to improve T cell transfection effeciency?

Anyone can tell me how to improve the transfection effeciency in old T cells that have been cultured for long time. I tried to transfect human PBMC derived T cells which have been cultured more...

07 August 2025 4,252 0 View

Why I get inconsistant Hazard Ratio (HR) analysis using SPSS?

Seeking expertise on the hazard ratio (HR) analysis. When I analyze all the 3 groups of data together, I get the Exp(B) as shown in second figure. However, if only the first and third groups are...

06 August 2025 582 2 View

Experimental variation of gene copy number measurement using qPCR?

I measured the gene copy number of a same sample (genomic DNA) twice last month and this month. The values are 3.8 and 5.9. I wonder whether this variation is normal. How large the experimental...

26 May 2025 8,705 1 View

Why the green signal of JC-1 do not decrease upon potential loss?

I am trying to use JC-1 for mitochondrial potential detection. What I understand is that the JC-1 is a ratiometric dye. When mitochondrial potential loss, JC-1 become less aggregated, and the...

13 December 2020 1,732 3 View

What happened to my fixed cell sample for mitochondria imaging?

I recently prepared some fixed cell samples for imaging. I need to observe the mitochondrial morphology. I overexpressed a GFP tagged protein, which localizes in the matrix of mitochondria. As is...

09 September 2020 7,970 6 View