Reamplification of Gel Purified PCR Product gives same non specific bands. How to get rid of non-specific band?

I did an overlap-extension PCR and got the following gel image (purple image + neb 1k ladder). The target band size is 1.9kb, which was the brightest band. Afterwrds, I cut the 1.9kb bands and...

09 September 2022 8,394 0 View