I can't find a blue plaque after second round of M13 amplification,with a plaque titering assay. Could any one help me to understand the problem?

I did the 2nd round amplification of M13 phage containing two control proteins A.Streptavidin B.N-acetyltransferases 1. After the phage titration i was not able to see any blue plaque on...

06 October 2015 1,669 2 View