Don A Delker

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Questions related from Don A Delker

I'm using DESeq2 to evaluate the effect of drug on gene expression from subjects with varying numbers of samples (2-5). Would this be biased?

I'm using DESeq2 to evaluate the effect of drug on gene expression from subjects with varying numbers of samples (2-5). Would this be biased? design attached

05 May 2016 6,916 5 View

Is it advisable to include 5' and 3' utr sequence when constructing an expression plasmid?

We are interested in looking at the overexpression of select transcription factors in cancer cell lines. Our RNA sequencing data from cancer tissue suggests there may be alternative splice sites...

12 December 2014 3,608 2 View

Is there an R statistical program for calculating differential gene expression with paired RNA-seq data?

Our prior DESeq anayses compares groups of samples but not does take into account biological hetergeneity between tumor/normal pairs.

10 October 2014 7,227 4 View