Dominika Pilat

2 Questions 1 Answers 0 Followers

Questions related from Dominika Pilat

How to eliminate dark background in DAB stains of mouse brain cuts?

Hi, I recently started doing a lot of IHC and I’ve been staining mouse coronal section (not in parafin) with 3D6 antibody to visualize plaques. My problem is that the background is too dark and...

03 December 2022 6,747 1 View

Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

01 October 2020 2,612 1 View