Are HEK293T cells able to express Tet-On regulated proteins without transactivator or Doxycycline under certain circumstances?

I tried to produce Lentiviruses using the plasmids pRSV-Rev, pCMV-VSVg and pMDLg-pRRE, as well as a plasmid containing TetOn-GFP. After transfection into HEK293T cells a lot of cells were...

26 October 2018 3,472 5 View