Dominik Alterauge

3 Questions 11 Answers 0 Followers

Questions related from Dominik Alterauge

Type I collagen Western Blot?

I would like to show type I collagen alpa2 (COL1A2) downregulation on protein level. Immunoblotting using 5 different antibodies didn't yield any bands at all (not even positive controls)....

17 August 2013 8,430 16 View

Differences in fluorescence of denatured lysozyme.

Upon denaturation of a protein, fluorescence intensity and wavelength (max) can change and I thought it would be the same for any kind of denaturation (chemical/thermal). But as I just learned...

12 April 2013 1,913 5 View

How to distinguish between the fluorescence of an excited dimer and a normal dimer?

I just got a weird, broadened emission band for my poly-ala-helix (21 residues incl. some arginines for solubility) which has two labels (Xanthone and Naphtaline) that I'm using for...

13 December 2012 5,954 24 View