Doctor Muhandas

3 Questions 9 Answers 0 Followers

Questions related from Doctor Muhandas

What is the best possible way to select ISSR primerS for any plant species ( No literature is available on molecular marker study of that species)?

I am interested in molecular markers study of cordyline spp. there is no literature available on ISSR ,SSR or even RAPD of cordylines. Can anyone help that how to select best primers?

06 March 2019 8,487 5 View

Purification of PCR product

Hello All, Can anyone have an experience to purify PCR product without using KITs suitable for ligation and cloning. If someone know any manual procedure please guide me about it. Thanks

15 February 2012 317 11 View

My question is that is there any way /method to check the function or activity of fruit specific promoter prior to transformation in plant?

I have isolated some fruit specific promoters by PCR amplification. On gel they are giving expected band size. I want to develop a gene cassette in which gene of interest will be under the control...

21 January 2012 1,308 5 View