Divyesh Patel

3 Questions 5 Answers 0 Followers

Questions related from Divyesh Patel

How to stain bacteria with propidium iodide to determine dead cells by flow cytomatory and microscopy?

I have to determine the dead cells in culture and I have propidium iodide in powder form and I have diluted 2mg with 2mL sterile filtered double Distilled water and to stain bacteria I use 10micro...

04 August 2020 9,023 4 View

How to stain and fix bacteria with presidium iodide for good results of Fluorescence microscopy ?

I am working with S.aureus bacterial strain and inducing cell death in it with antibacterial treatment. I want to compare my control and treated sample in Flow cytometry and fluorescence microscopy.

27 January 2020 2,309 3 View

Which dilution plate can be considered if CFU is coming under countable range of 30-300 for two different dilution?

i am working with microbial suspension in broth and to determine the concentration of bacterial culture i have done serial dilution till 10^9 where 10^8 gives 38 colonies and 10^7 gives 179...

29 April 2019 5,239 8 View