Divyank Mahajan

4 Questions 7 Answers 0 Followers

Questions related from Divyank Mahajan

How to measure expression levels of a gene in two different cell lines?

If one needs to compare the expression levels of a particular gene experimentally (molecular method, not omics approach), can there be a reliable method to measure it? What kind of control can be...

12 December 2019 7,732 3 View

While doing Gene Set Enrichment Analysis, should one separate up- and down-regulated genes?

If we have a list of differential proteins from proteome data-sets, should we use all the differential genes together while running the analysis? Or, should those be run separately? Additionally,...

05 May 2018 8,889 3 View

Do we need to calculate qPCR EFFICIENCY for all the 'Kinds' of test samples?

If one has multiple test conditions vs a Control condition, Should one calculate qPCR Efficiency for all of them (For eg., for 5 time-points X 5 perturbations = 25 samples) ??? & with multiple...

04 April 2015 7,908 4 View

Is DNAase treatment after RNA Extraction NECESSARY if it is to be used for cDNA preparation and qPCR thereafter?

Mammalian Cells.

02 February 2015 3,314 6 View