Divyank Mahajan

5 Questions 4 Answers 0 Followers

Questions related from Divyank Mahajan

A basic question - How do you define a "cell-state" or a cell-subtype?

Analyzing omics results from a single-cell genomics/single-cell spatial genomics experiment would reveal that there are these many cell clusters present/found. There will be cases, where the data...

10 January 2024 4,412 2 View

How to measure expression levels of a gene in two different cell lines?

If one needs to compare the expression levels of a particular gene experimentally (molecular method, not omics approach), can there be a reliable method to measure it? What kind of control can be...

03 December 2019 6,511 3 View

While doing Gene Set Enrichment Analysis, should one separate up- and down-regulated genes?

If we have a list of differential proteins from proteome data-sets, should we use all the differential genes together while running the analysis? Or, should those be run separately? Additionally,...

04 May 2018 7,419 3 View

Why does reverse transcriptase (RT) enzyme NOT amplify cDNA it made itself ?

As reverse transcriptase has both RNA-dependent DNA polymerase as well as ssDNA-dependent DNA ploymerase activity, then, why, while making RNA to cDNA, we do not see an amplification of cDNA formed?

25 March 2015 9,453 3 View

Can we use trypsin to passage cells during SILAC based proteomic experiments?

Thank you.

17 March 2015 8,446 3 View