Divya Tarwadi

2 Questions 6 Answers 0 Followers

Questions related from Divya Tarwadi

Why osmotic balance of cell is important during plasmid isolation?

i have performed alkaline lysis method for plamid isolation. 50mM glucose is added in alkline solution I for osmotic balance. My question is why cant we just bust cell instead of cell lysis for...

12 December 2019 3,566 4 View

Can monomeric protein form dimer during protein expression?

I have express protein with nisin expression system, which is of 37 kDa. but i got 74 k Da band in SDS PAGE which is twice then my protein of interest.which is unlikely possible in denaturing...

10 October 2019 8,794 8 View