Divay Chandra

3 Questions 2 Answers 0 Followers

Questions related from Divay Chandra

Does any one have experience with the BlotCycler automated western blot processor from Precision Biosystems?

Is this western blot processor reliable and a good buy? Our lab does a lot of western blotting.

06 October 2016 2,014 0 View

LDH release assay in THP-1?

I want to determine cytotoxicity of oxidized LDL in THP-1 macrophages in culture (adherent cells after PMA treatment). Is the LDH release assay a good choice. Can I collect and freeze the...

24 September 2016 3,283 2 View

What is the reason for NFKB activation in THP-1 at baseline?

I am growing THP-1 cells. After 48 hours of PMA exposure (50ug/ml) and two days of rest with RPMI 1640 medium with 10% FBS I see phosphorylation of the P65 subunit of NFkB (without any stimuli...

28 January 2016 720 5 View