Why am I unable to detect IFN-γ in CD8+ and CD4+ T cells from tumor tissue using flow cytometry?

I am analyzing CD8+ and CD4+ T cells in tumor tissue using flow cytometry but cannot detect IFN-γ. I did not use any stimulation. Reagents I used were cytofix, perw/wash buffer, cell staining...

26 February 2025 3,096 4 View