Diraviyam Thirumalai

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Questions related from Diraviyam Thirumalai

Could anyone please help me to amplify a target gene (Clostridium difficile -Toxin A and Toxin B) by using a PCR method?

I would like to amplify two toxin genes of C. difficile followed by expressing toxin A and B using bacterial expression system. In which we started with the amplification of gene using PCR, we...

25 June 2014 2,807 2 View

Anyone can guide me on how to develop antibiotic associated diarrhea (AAD) mouse model?

We would like to develop AAD mice model in order to determine the effect of antibody therapeutics against AAD in mice. In this process, we selected C57BL/6 mice and treated with antibiotic...

25 June 2014 8,187 2 View