5 Questions 19 Answers 0 Followers
Questions related from Dipendra Kumar Ayer
Q1. Katsuyama et al. (2009) had designed the primers for the full-length cDNA of CURS2 using a pair of primers, 5′-GCTAATCAGTCAATCCAGATGG-3′ and 5′-CGTCTATCGATTGATCGATCGTG-3′. These primers were...
20 September 2017 9,893 4 View
I have performed qRT-PCR in Bio-Rad CFX96. The melting curve result shows two peaks in some samples within replicates but not all. What might be the cause? And, based on these attachments, would...
27 March 2017 2,875 6 View
I measured the quality of cDNA using the Nanodrop. I found similar absorbance for No Template Control also. Although the A260/280 ratio is around 1.8, even no template control PCR product is also...
06 February 2017 6,699 5 View
I used 0.8% agarose gel for PCR products and 1-1.2% gel for genomic DNA made with 1x TE buffer. But, I am confused that % increase in gel concentration decreases the pore size and fragments to be...
24 January 2017 6,340 18 View
I used Qiagen RNeasy Plant Mini Kit to isolate RNA from Turmeric Rhizome (6 months old) and used tertiary rhizomes as a sample for RNA isolation. I used two varieties (First Three columns...
21 January 2017 7,642 13 View
