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Hi, I was doing protein purification today and i observed that my niNTA beads turned brown after i bound my lysate with the beads. I eluted my protein but still the color of the beads were...
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I am working with a protein-12kda; my vector contains 10X His tag My protocol for purification- NOTE- this is from 1 ml sonicated supernatant, used 100ul NiNTA beads. After I collect Flow...
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Can anyone help me.. Is there any kit for cDNA Library construction for membrane proteins? I want to perform mbsus assay (Mating Based Split-ubiquitin Assay ) which is a alternative method for...
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