Dipanshu Ghosh

6 Questions 2 Answers 0 Followers

Questions related from Dipanshu Ghosh

Can somebody suggest me where can I get/purchase Picrorhiza kurrroa seeds from?

I am willing to do some kind experiments on Picrorhiza kurroa and for the same I need seeds.

13 January 2022 9,853 2 View

How to increase the value of A 260/230 value of eluted DNA?

When I elute out DNA for cloning purpose, on measuring nano drop reading I get very low value of A 260/230 (it's like 0.04 or 0.08 and so). Even after repeated washing of column this problem...

09 January 2022 112 5 View

I am facing problem in plasmid isolation. How to fix it?

I am getting appritiable growth of bacteria in antibiotic medium. On isolating plasmid by conventional method or by kit I am getting either very little amount of plamids or no plasmids or...

27 May 2021 8,685 5 View

How does SDS add uniform negative charges to the protein?

As we know when we treat a protein with SDS, it breaks non covalent bonds to form primary structure. But how it adds negative charges uniformly on all constituent amino acids?

24 December 2020 1,825 6 View

Why is the solution turning blue when I add 95% ethanol to commisse brillant blur G250?

I am trying to prepare Bradford reagent in lab. When I'm adding 95% ethanol to commisse blue G250 dye to dissolve it, the solution is turning blue. Does it indicate to any problem in Bradford...

01 January 1970 1,259 4 View

I can't clone a 3kb dna into pGEMT EASY.

I want to clone a 3kb DNA fragment into pGEMT EASY. On gel I can see 3kb band but I can't clone it into pGEMT EASY. Can anyone suggest how I can troubleshoot?

01 January 1970 9,502 3 View