Diogo Trigo

2 Questions 4 Answers 0 Followers

Questions related from Diogo Trigo

Is it possible to get signal for Actin on a western blot prior to incubating with a-actin antibody?

I have a western blot which I incubated with an antibody anti-ADH 7. I had a faint band appearing at approx 42 kDa. When I incubated with anti-actin afterwards, a band at approx 42 kDa appeared,...

02 February 2016 2,429 2 View

What protein control to use in purified mitochondria western blot?

I have purified mitochondria to run in a western blot, but I am not sure what protein control I should use, as I don't think that mitochondria express actin. Any suggestions?

09 September 2013 4,791 55 View