Diogo Trigo

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Questions related from Diogo Trigo

Is it possible to get signal for Actin on a western blot prior to incubating with a-actin antibody?

I have a western blot which I incubated with an antibody anti-ADH 7. I had a faint band appearing at approx 42 kDa. When I incubated with anti-actin afterwards, a band at approx 42 kDa appeared,...

05 February 2016 9,781 2 View

What protein control to use in purified mitochondria western blot?

I have purified mitochondria to run in a western blot, but I am not sure what protein control I should use, as I don't think that mitochondria express actin. Any suggestions?

06 September 2013 4,571 54 View