What is the best optical density equivalent to 0.5 MacFarland standard for Pseudomonas in MIC determination?

I tried to use OD of 0.1 (660nm) as standard. While on plate count it came different than my expectation. Then, I tried OD 0.08, yet I did not get the actual number 5x105  cfu/ml on plate count on...

12 November 2015 3,125 12 View