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Questions related from Dina M Mofed
I have a question. I need to cotransfect two plasmids: plasmid X and plasmid y According to the manufacturer's protocol for 24well plate I should take 0,5ug of DNA and 2ul of lipofectamine. Do...
23 March 2021 6,200 4 View
Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks
22 December 2020 1,259 2 View
“I have two questions , thw first one is how can I increase growth of competent cells DB3.1 in LB btoth media containing amp / kanamycin The second , I use vector BACMAM pCMV dest which contains...
20 July 2019 7,866 1 View
Hi all, I have a problem with Huh7 cell line where, I maintained it on DMEM media ( high glucose ), FBS 10 % and after first passage, most of the cells dead after passage in new flask and the...
13 February 2019 3,518 1 View
