Dina Khalid

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Questions related from Dina Khalid

Help optimize multiplex PCR: COVID-19, Change dye?

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange? The curve by B in Hex starts...

27 May 2020 7,664 0 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

11 May 2020 7,977 3 View