Dimitri Janin

3 Questions 2 Answers 0 Followers

Questions related from Dimitri Janin

Loss of purified protein during concentration step ?

Hi all, I am trying to concentrate my purified protein using Corning 30kDa concentrators and my protein is about 78 kDa. After elution I have 1.5 mg of protein total in my fractions but after few...

28 September 2020 7,096 5 View

How to store GSH elution buffer for GST-tagged proteins purification ?

In order to keep my Tris 20 mM GSH pH8 elution buffer active for as long as possible I would like to know if I could prepare a large quantity and freeze it? (how much time can I freeze/thaw it?)...

20 February 2020 8,449 3 View

How to store GSH elution buffer for GST-tagged proteins purification ?

In order to keep my Tris 20 mM GSH pH8 elution buffer active for as long as possible I would like to know if I could prepare a large quantity and freeze it? (how much time can I freeze/thaw it?)...

02 February 2020 10,037 3 View