Why protein after Ni-NTA purification shifted to higher molecular weight in SDS-PAGE?

I have cloned Hemagglutinin (HA1 only) with 6xHis on its C-terminus into pet23C and expressed in E.colli (Bl21(DE3)) and then purified on Ni-NTA column, but my protein after purification had...

02 February 2020 5,656 5 View