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Questions related from Dilip Sharma
In a HPLC chromatographic Method diluent pH is different from mobile Phase pH. What is the impact on chromatography for this condition. Kindly suggest and provide any lit. if any.
19 September 2024 6,637 0 View
In a combination drug product related substances test we are generating impurity profile. two claim is there:- 1. 100 mg 2. 5 mg Now on the calculation of unknow impurity we are calculating the...
12 September 2024 5,154 1 View
Gradient program containing mobile phase A with 0.1 % Trifluoroacetic acid in water and B with 0.1% TFA with Acetonitrile. Unknown peak elute at same retention time at main component in blank...
12 June 2023 6,556 1 View
Is there any tool to calculate Column CDF value.
07 December 2022 5,925 3 View
Is there any possibility to analyse Pancreatin enzyme on HPLC without any treatment? Size exclusion UV chromatography seperate the 11 peak in Pancreatin enzyme. My concern is Lipase, amylase and...
25 December 2019 10,030 3 View
Dear all, I develop a method for my product, everything was perfect but at last there is peak in BLANK at the component RT. sometime it happen only like a baseline drife. But it comes, where 75...
30 December 2016 2,506 14 View
With regard, i want to know that what is the basic behind the capacity factor. what is the role of capacity factor in analysis? as we can say migration rate of analyte on column about this, but...
04 May 2016 6,157 12 View
With regard, My query is about IR analysis, in the IR region of 2500 to 3400 cm-1, the functional group response is very very low. (Peak of -OH group and amine and other group) Peak intensity in...
04 May 2016 8,642 7 View
I'm using Chiralpak IA column for my analysis. initially there was good resolution in my analysis, but followed by time the both peak in racemic merged. My mobile phase is only n-Hexane:IPA...
17 September 2014 2,979 9 View
