Dilek Cansu Gürer

2 Questions 7 Answers 0 Followers

Questions related from Dilek Cansu Gürer

How can I increase cDNA concentration for qPCR?

I cannot see the expression of my long non-coding RNA in qPCR but when I overexpress this lncRNA I have Cq values about 11 so I think my primers are working. I may be wrong but I think my cDNA...

04 April 2018 3,285 14 View

Does anyone know why hela cells change shape and look like more fibroblast?

Hello everyone. In my experiment in 24wp some of the wells look like more fibroblast than hela. I used RPMI with 10% FBS and no antibiotics. Do I have a contamination? or are my cells dead? The...

03 March 2018 834 3 View