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We are trying to create an SMR for our ICU network. We calculated the predicted mortality for all the patients for six months and plotted an ROC for the prediction tool used. However from the...
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I am trying to do HIF1 westerns in mouse brain tissue. 1. Is the nuclear extraction protocol same as that of the other tissues? I am getting low yield of protein when I use the ones for liver and...
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For HIF1 alpha western blot, while doing the nuclear extraction, on following the volumes in the protocol (100ug tissue and 300uL solute) protein concentration in the samples prepared is too low....
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