Dileep Ahari

2 Questions 5 Answers 0 Followers

Questions related from Dileep Ahari

What are possible reasons for not getting exact molecular Mass of protein in mass spectroscopy (MALDI)?

I'm purifying a protein using bacterial expression system. Purification is done using affinity chromatography. While on the SDS PAGE it shows band at expected mass (17 kDa) but molecular mass...

26 June 2021 7,961 2 View

What will be the effect of incomplete labeling of the acceptor in a FRET experiment?

Labeling of my protein with donor is 1 while labeling of my protein with acceptor is 0.8. Donor and acceptor are present on different set of proteins and I'm trying to look at the interaction...

04 March 2015 632 4 View