Diksha Rani

2 Questions 2 Answers 0 Followers

Questions related from Diksha Rani

How to run insoluble or pellet fractions in Western blotting?

Hi everyone, I’m working with samples that have a significant insoluble fraction after lysis and centrifugation. I would like to run the pellet (insoluble) part on a Western blot, either because...

02 July 2025 4,149 1 View

RT-PCR Not Working – Could My Primer Dissolution or Handling Steps Be the Issue?

Hello everyone, I’m facing an issue where my PCR isn’t working, and I’m trying to troubleshoot whether the problem lies in how I handled the lyophilized primers. Here is the step-by-step protocol...

11 June 2025 4,763 8 View